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Objective: This report evaluated the efficacy of three brushes and one biofilm disclosing agent in complete denture cleansing. Save game fm 2007 editor. Methods: Twenty-seven wearers of maxillary dentures were distributed into three groups and received different brushes: Oral B40, conventional toothbrush (Oral B); Denture, denture-specific brush (Condor); Johnson & Johnson, denture-specific brush (Johnson & Johnson). The 60-day experimental period was divided into two techniques: I - brushing (brush associated with a paste - Dentu Creme, Dentco) three times a day; II - brushing and daily application of 1% neutral red on the denture internal surface. Biofilm quantification was carried out weekly and the areas with dye biofilm were obtained by means of Image Tool 2.02 software. INTRODUCTION Although many reports evaluate the efficacy of denture cleansers, surveys point out that removal of denture biofilm by denture wearers is precarious–. Deficient patient orientation, inadequate divulgation of specific denture cleanser materials, intrinsic characteristics of prosthetic appliances and deficient manual dexterity of elderly people have been indicated as causes of poor denture hygiene. Biofilm removal can be obtained by means of mechanical methods (brushing and ultrasonic devices) associated with chemical methods (alkaline peroxide and hypochlorite, acids, enzymes and disinfectants); among them, brushing is the most common method applied for routine denture biofilm control,.